Generator

Part:BBa_K1833005

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-16)

pT7-Phage 434 repressor N-terminus

This part generates the DNA binding domain of the phage 434 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit the 2015 Pitt iGEM page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None